Extended Data Fig. 2: Changes in PPs induced by one 3-day switch to FD.
From: Short-term dietary changes can result in mucosal and systemic immune depression
(a–d) UMAP (A) and related clusters (B) and parameters (C) calculated on PP CD3+CD4+ viable T cells isolated from RD- and FD-fed mice. (d) Frequencies (left) and numbers (right) of PD1+CXCR5+GL7+ and PD1+CXCR5+GL7− TFH cells among CD3+CD4+ T cells in PPs. (e) Frequencies (left) and numbers (right) of IgA+GL7− (non−GC) and IgA+GL7+ (GC) cells among B220+ B cells in PPs. (f) Genetic setup of IL-17A fate-mapping reporter mouse. (g) Cell numbers per PP of exTH17, TH17 and IL-10-secreting TH17 cells. (h) Dietary intervention schematic. (i–j) Frequencies (I) and numbers (J) of SILP exTH17 and TH17 cells among CD3+CD4+ T cells, after fasting and mucus wash. (k) Pie charts depicting frequencies of IL-17A-producer cells in PPs. (l) Representative dot plots of ROR-γt versus IL-22 gated on SILP Lin-CD45+CD127+ viable cells. (m) Frequencies (left) and numbers (right) of SILP ILC3s and IL-22-secreing ILC3s. (n) Normalized counts of fut2 gene in ileum cells, as measured by RNA bulk-seq. (o) Dietary interventions and S. Typhimurium infection model. (p, q) Body weight loss and CFUs of S. Typhimurium in SI luminal content and liver. (r) Graph summarizing CFUs in SI luminal content, Shannon’s diversity and il17a/f levels in ileum and PPs as per experimental plan in (O). Data in (A-C) are from one experiment representative of 2 (n = 5 each). Data in (D-E) are a pool of 2 experiments (n = 9 or 10 each). Data in (G) are a pool of 2 experiments, representative of 4 (n = 9, 7, 8 or 5). Data in (I-J) are from 2 experiments (n = 7 or 6). Data in (M) are a pool of 2 experiments (n = 7 each). Data in (N) are from one experiment (n = 3 each). Data in (P) are from one experiment (n = 5 each), representative of 2. Data in (Q) are a pool of 2 experiments (n = 11, 10 or 12). Data are shown as mean ± SEM or median (Q). P.values have been determined by two-tailed non-parametric Mann-Whitney test (B, D-E, G, I-J, M), Brown-Forsythe and Welch ANOVA test (P) or Kruskal-Wallis test with Dunn’s multiple comparisons test (Q).
