Extended Data Fig. 5: The L1 domain of TCF-1 modulates binding and transcriptional outcomes in early T cell development independent of chromatin accessibility.

a) SeqLogo depicting top enriched motifs from de novo HOMER motif analysis of differentially accessible ATAC-seq peaks in WT vs. EV, ΔL1 vs. EV, and WT vs ΔL1 transduced DN1 and DN2s with non-differential peaks as background. P values are calculated using a hypergeometric test. b) Venn-diagram representing TCF-1 CUT&RUN experiments and associated unique and overlapping WT TCF-1 and ΔL1 binding events in DN1 and DN2s. c) Principal component analysis of TCF-1 CUT&RUN and chromatin accessibility measurements in DN1 and DN2s. Counts of ATAC-seq and TCF-1 binding in CUT&RUN measurements were generated across the union of all peaks across all ATAC-seq and CUT&RUN conditions. d) SeqLogo depicting top enriched motifs from de novo HOMER motif analysis of L1 dependent and independent binding events in DN1 and DN2s compared to randomly generated background. P values are calculated using a hypergeometric test. e) Heatmap depicting TCF-1 binding events measured by TCF-1 CUT&RUN and chromatin accessibility in DN1 and DN2s at differentially accessible peaks open in WT DN2 vs. DN1s.