Extended Data Fig. 3: Targeting microglial APOE4 restores neurodegenerative microglia in APP/PS1 mice.
From: APOE4 impairs the microglial response in Alzheimer’s disease by inducing TGFβ-mediated checkpoints
a, UMAP plot of unsupervised seurat clusters from scRNAseq analysis of brain cells isolated from APP/PS1:APOE4-KI and APP/PS1:APOE4-cKO mice (n = 2). b, UMAP plot of labelled cell types. c, Cell-specific genes used to identify cell types from seurat clusters. d, UMAP plot of microglia/myeloid cells re-clustered. e, Feature plot showing expression of border-associated macrophages genes. f, Heat map of DEGs representing the microglia/myeloid cluster. DEGs were identified using FindAllMarker Seurat function (P < 0.05). g, Feature plot showing co-expression of Lgals3 and Clec7a microglia. h, Ridgeplot of Clec7a and Lgals3 in APOE4-cKO and APOE4-KI in MGnD microglia showing gene expression cut-off for Clec7aHi (2.65) and Lgals3Hi (1.68) microglia.
