Extended Data Fig. 4: Deletion of microglial APOE4 alleviates lipid dysregulation in APP/PS1 mice.
From: APOE4 impairs the microglial response in Alzheimer’s disease by inducing TGFβ-mediated checkpoints
a, Heat map of lipids significantly altered by genotype in microglia isolated from APOE3-KI, APOE4-KI, APP/PS1:APOE3-KI, and APP/PS1:APOE4-KI. DEGs were identified using DESeq2 analysis with an LRT (n = 2 mice, P < 0.05). b, Confocal images of Plin2, Iba1 and HJ3.4B in APP/PS1:APOE3-KI, APP/PS1:APOE3-cKO, APP/PS1:APOE4-KI, and APP/PS1:APOE4-cKO mice. Scale bar: 50 μm. c, Quantification of Plin2+ area in Iba1+ cells (n = 15 cells from APP/PS1:APOE3-KI mice, n = 8 cells from APP/PS1:APOE3-cKO mice, n = 24 cells from APP/PS1:APOE4-KI mice, n = 16 cells from APP/PS1:APOE4-cKO mice). One-way ANOVA. Data presented as mean ± s.e.m.
