Extended Data Fig. 7: No expression of MNP-2 surface phenotype with naive CD8 T cell activation. | Nature Immunology

Extended Data Fig. 7: No expression of MNP-2 surface phenotype with naive CD8 T cell activation.

From: Trimodal single-cell profiling reveals a novel pediatric CD8αα+ T cell subset and broad age-related molecular reprogramming across the T cell compartment

Extended Data Fig. 7

(a) Surface protein marker profiles of naive and MNP-2 CD8 T cells. (b) Naive CD8 T cell activation experiment with TCR stimulation (anti-CD3/anti-CD28 beads (0.5 beads per cell)). Cells were assessed over a 7-day time course for MNP-2 surface markers (CD8-beta, CD244, CD11b, IL21R) and activation markers (CD69, CD25, CD71) by flow cytometry. (c) Representative plot of MNP-2 cells delineated by CD8-betaLow and CD244high co-expression pre- and post-isolation, as well as over a 7-day time course post-TCR stimulation. (d) Frequencies of CD8-betaLowCD244high MNP-2 cells over the TCR stimulation time course (n = 4–7 donors). Results are from three independent experiments. P-values were determined by one-way ANOVA with Holm-Sidak’s multiple comparisons test. ****P < 0.0001. Tukey’s box plots with median with 1st and 3rd quartiles shown. (e) Representative histograms of activation markers and (f) frequencies of positive cells for each activation marker over the TCR stimulation time course (n = 4 donors). Mean +/− sem.

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