Fig. 7: IL-21-induced responses in pediatric CD8+ T cell subsets. | Nature Immunology

Fig. 7: IL-21-induced responses in pediatric CD8+ T cell subsets.

From: Trimodal single-cell profiling reveals a novel pediatric CD8αα+ T cell subset and broad age-related molecular reprogramming across the T cell compartment

Fig. 7

a, Overview of a fixed CITE-seq experiment for IL-21 stimulation. b, RNA-based UMAP plot of unstimulated and IL-21 (50 ng ml−1, 4 h)-stimulated pediatric CD8+ T cells (n = 4 pediatric donors). Select stimulated subsets are indicated. Mem, memory; unstim, unstimulated. c, Select gene expression indicative of IL-21R signaling in IL-21 stimulation, shown in RNA-based UMAP plots. Pseudobulk RNA expression in naive, MNP-2 and memory CD8+ T cells after IL-21 stimulation is shown to the right of each UMAP plot. exp, average expression level. d, Comparison of DEGs across each subset of IL-21-stimulated and unstimulated CD8+ T cells. e, Violin plots of select DEGs from CD8+ T cell subsets after stimulation with IL-21.

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