Extended Data Fig. 3: Loss of SIRPa does not alter macrophage differentiation.
From: CD47 masks pro-phagocytic ligands in cis on tumor cells to suppress antitumor immunity
a, RNA sequence of Sirpa-/- vs WT BMDMs was analyzed. b, expression of various cell surface markers (red lines) in WT and Sirpa-/- BMDMs assayed by flow cytometry. Filled curves, Ctrl IgG. c, expression of various cell surface markers (red lines) in WT and Sirpa-/- BMDMs from a second Sirpa-/- C57BL/6 mouse strain (#54) assayed by flow cytometry. Filled curves, Ctrl IgG. d,e, Phagocytosis of L1210 cells (d) and Raji cells (e), expressing or not expressing CD47, by WT or Sirpa-/- BMDMs as in Fig. 2a. All data are means ± s.e.m. ns, not significant; **p < 0.01, ***p < 0.001, and ****p < 0.0001. 3 pairs of mice were studied in one experiment (a). Flow cytometry profiles are representative of 3 (b,c) independent experiments. Results are pooled from a total of 3 (d,e) mice studied in 3 independent experiments. Each symbol represents one mouse.
