Extended Data Fig. 5: CD47 interacts in cis with SLAMF7.
From: CD47 masks pro-phagocytic ligands in cis on tumor cells to suppress antitumor immunity
a, Expression of Flag or CD47 (red lines) on L1210 cells and Cd47-/- L1210 cells, expressing or not expressing mCD47-Flag, assessed by flow cytometry. Permeabilization was used for the Flag staining. Filled curves, Ctrl IgG. b, Means of the normalized total ion current (TIC) for the potential interactors of CD47. c, Expression of hCD47 (red lines) on WT or CD47-/- 293 T cells was assessed by flow cytometry. Filled curves, Ctrl IgG. d, Fluorescence intensity of Dye 647 and Dye 547 in CD47-/- 293 T cells, transfected with tagged versions of mCD47 and mSLAMF7 and treated with or without Ctrl IgG, mCD47 mAb and mSLAMF7 mAb, assayed by flow cytometry. e, Binding of soluble mSIRPα-Fc fusion protein and expression of mCD47 on transfected CD47-/- 293 T cells assessed by flow cytometry. f, Binding of soluble mSLAMF7-Fc fusion protein and expression of mSLAMF7 on transfected BI-141 cells assessed by flow cytometry. g,h, Representative confocal microscopy images (g) and compiled data from 18 cells in 3 independent experiments (h) of FRET assays as in Fig. 4d,e. All data are means ± s.e.m. ns, not significant; ****p < 0.0001. Flow cytometry profiles are representative of 3 (a,c-f) independent experiments. Results are representative of 3 (g) independent experiments. Results are pooled from 3 (b,h) independent experiments.
