Extended Data Fig. 3: Targeting Tle3 promotes T_CM- but diminishes T_EM-chracteristic molecular features.

a. Phenotypic analysis of WT and Tle3^–/– memory CD8⁺ T cells at ≥30 dpi based on select cell surface markers. Based on scRNA-seq analysis, Klrg1 and Cx3cr1 transcripts were preferentially detected in WT T_EM compared to WT T_CM cells (Fig. 2b, c). While these features were retained in Tle3^–/– memory CD8⁺ T cells, their transcript levels were lower in Tle3^–/– T_EM cells compared to their WT counterparts (Fig. 2h). These changes were validated on the protein level with flow cytometry analysis. b. Gating strategy for cell sorting of WT and Tle3^–/– T_CM and T_EM cells from the immune mice at ≥30 dpi. The various changes in KLRG1 and CX3CR1 protein expression due to Tle3 deficiency (a) may not accurately reflect the composition changes in Tle3^–/– memory CD8⁺ cell pool. To avoid potentially confounding effects caused by the differential dependence of selected cell surface markers on the expression of Tle3, the CD62L-based classical definition of T_CM and T_EM cells was used here for cell sorting. c. Key comparisons for analysis of transcriptomic and ChrAcc changes in this study. d. Volcano plots showing DEGs between WT and Tle3^–/– T_EM cells (left) and those between WT and Tle3^–/– T_CM cells (right) by the criteria of ≥1.5-fold expression changes, FDR < 0.05, and FPKM ≥ 0.5 in the higher expression condition. Values in the plot denote DEG numbers in each pairwise comparison.