Fig. 7: Lineage tracing confirms distinct cDC2A and cDC2B ontogenetic lineages.

a, Representative flow cytometry plots of the expression of RFP and eGFP on Siglec-H⁺ and Siglec-H⁻ pre-cDC2s and percentage of Siglec-H-RFP⁺ and LysM-eGFP⁺ cells among pre-cDC1s and Siglec-H⁻ or Siglec-H⁺ pre-cDC2s from the bone marrow of SigH^RFPLyz2^eGFP mice. Pre-cDCs were identified using the UMAPs as in Extended Data Fig. 7b–d. b, Representative UMAPs (concatenated spleen, MLN, lung and liver) generated on CD11c⁺Lin⁻ cells using CD11c, MHC-II, CD26, CD64, CD88, XCR1, SIRPα, Esam, CLEC12A, CD11b, CD43, CD135, CD117, Ly6C and CD8α as in Extended Data Fig. 4c–e, overlaying RFP^hi and eGFP^hi cells in cDC2As and cDC2Bs and CD8α⁻ or CD8α⁺ pre-cDC2s. c, Percentage of RFP⁺ or eGFP⁺ cDC1s, cDC2As, cDC2Bs and pre-cDC1s, and CD8α⁻ pre-cDC2s or CD8α⁺ pre-cDC2s identified using the UMAPs as shown in Extended Data Fig. 4c–e from the spleen, MLN, lung and liver of SigH^RFPLyz2^eGFP mice. Gates for RFP⁺ and GFP⁺ cells were set using WT mouse cell counterparts. Each dot represents one mouse (n = 5); data are from one of two experiments (mean ± s.e.m.). A one-way ANOVA (with Tukey correction) was used for comparison. P values are indicated above the graphs.