Extended Data Fig. 9: Loss of baseline JAK-STAT signaling outside of the in vivo tissue context.
From: JAK-STAT signaling maintains homeostasis in T cells and macrophages
(a) Average sample-to-sample Spearman correlation among homeostatic, context-deprived and stimulated immune cells, visualizing the effects of ex vivo culture on wildtype immune cells. The average correlation among samples under homeostasis is shown as baseline indicated by a black line. (b) SingleR similarity scores110 comparing cultured and homeostatic cells to external reference profiles from the ImmGen consortium111. (c, d) Inferred receptor-ligand interactions of T cells and macrophages in single-cell transcriptome data from Tabula Sapiens. Examples of ligand-receptor interactions (c) between CD8+ T cells (left), macrophages (right) and various splenic cells (rows) (two-sided random sampling, corrected for multiple comparisons), and the total number of receptor-ligand interactions inferred from Tabula Sapiens (d). padj: adjusted p-value.
