Fig. 5: Epigenome effects of JAK-STAT mutants in homeostasis.
From: JAK-STAT signaling maintains homeostasis in T cells and macrophages
a, Outline of the analysis dissecting JAK-STAT modulation of the epigenome (based on ATAC-seq data) and transcriptome (based on RNA-seq data). b, Genome browser tracks showing chromatin accessibility profiles for the promoter regions of the Stat5a gene, the macrophage marker gene Cd14 and the T cell marker gene Cd28. c, Transcription factor footprinting analysis, showing differential chromatin accessibility footprints for certain JAK-STAT mutants and cell types. Shaded areas indicate the standard error. d, Bar plots showing the percentage of genes and genomic regions affected by transcriptome and epigenome changes upon JAK-STAT modulation (relative to the number of all tested genes or genomic regions), as well as the Pearson correlation between transcriptome and epigenome changes (log2FCs of gene expression versus chromatin accessibility of the corresponding gene promoter across all tested genes). Group annotations (in blue) were manually assigned based on qualitative similarities in the transcriptome and epigenome effects. e, Scatterplot of log2FCs for transcriptome versus epigenome changes upon STAT3 and STAT5 knockout.
