Extended Data Fig. 6: Related to Fig. 8.
A) Predicted ligand–receptor interactions between MSC cluster from Fig. 7 and neutrophil clusters of MM patients after treatment. Expression magnitudes and interaction specificity values were calculated using the statistical framework of liana22 B) Predicted ligand–receptor interactions between MSC cluster from Fig. 7 and neutrophil clusters of MM patients at diagnosis. Expression magnitudes and interaction specificity values were calculated using the statistical framework of liana22 C) Transcription of IL1B and TNFSF13B comparing non-cancer controls to patients after consolidation therapy in the neutrophil object generated using Harmony (with sample ID, sex and sequencing batch as co-variates) D) Transcription of CCL2, CCL3, CXCL2 and IL1B in macrophages comparing those of treated MM patients with those of NDMM patients and controls E) BAFF protein in BM plasma of N = 18 controls, N = 24 NDMM patients and N = 18 patients after induction treatment F) BAFF protein in BM plasma of N = 52 patients after consolidation therapy, split by MRD status post-consolidation G) BAFF protein in BM plasma of N = 52 patients after consolidation therapy, split by IMWG Uniform Response criteria therapy response78 H) IL-1β protein in BM plasma of N = 52 patients after consolidation therapy, split by MRD status post-consolidation I) IL-1β protein in BM plasma of N = 52 patients after consolidation therapy, split by IMWG Uniform Response criteria therapy response78. PR, partial response. VGPR, very good partial response. CR, complete response. sCR, stringent complete response. Data are presented as mean ± SEM. Significance was calculated in B-F, using the Mann–Whitney U test (two-tailed), *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001.
