Extended Data Fig. 6: LpIMB19 induces a mild inflammatory phenotype in small intestinal macrophages.

B16F10 mouse melanoma cells (2x10⁵/mouse) were implanted subcutaneously and LpIMB19 was administered (1x10⁹ CFU/mouse, oral gavage) once every 3 days, starting from day 0 till the end of the study. Intestine and tumor-infiltrated immune cells were isolated on day 20 and subjected to flow cytometry analysis. a-c, Frequency of IFN-γ⁺, IL-2⁺ and TNF⁺ cells in CD8 T cells, isolated from SI-LP (a), Colon (b), and melanoma (c) (n = 7 mice for PBS and n = 8 mice for LpIMB19). d-e, Representative flow diagram of CX3CR1⁻Ly6C^int-lo macrophages (d) and frequency of CX3CR1⁻Ly6C^int-lo, CD86⁺, iNOS2⁺, and CD206⁺CX3CR1⁻Ly6C^int-lo macrophages (e) in SI-LP (n = 11 mice). f-g, Representative flow diagram of CX3CR1⁻Ly6C^int-lo macrophages (f) and frequency of CX3CR1⁻Ly6C^int-lo, CD86⁺, iNOS2⁺, and CD206⁺CX3CR1⁻Ly6C^int-lo macrophages (g) in colon (n = 11 mice). h, Representative flow diagram of CX3CR1⁻Ly6C^int-lo macrophages in tumors. i, Representative flow histograms (top) and frequencies of iNOS2⁺, CD86⁺, and CD206⁺ in CX3CR1⁻Ly6C^int-lo macrophages in melanoma (n = 7 mice for PBS and n = 8 mice for LpIMB19). Results are pooled from two (a-c, i) or three (e, g) independent experiments. Data are mean ± SEM (a-c; e, g, i). Statistical analyses were performed using two-tailed unpaired Student’s t-test (a-c; e, g, i). SI-LP, small intestine lamina propria.

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