Extended Data Fig. 6: SAA regulate dendritic cell infiltration into primary tumors.
From: Hepatocytes coordinate immune evasion in cancer via release of serum amyloid A proteins
a) Saa+/+ (n = 3) or Saa−/− (n = 4) mice were orthotopically injected with 5e5 PDA.69 cells. Twenty days later, tumors were harvested and analyzed by CyTOF. tSNE plots from 5000 CD45+ downsampled cells were generated and clusters identified using R software. b) Heat map displaying marker expression for each cluster. c) Expression of dendritic cell markers on all clusters. d) DC frequency measured by manual gating. For a-d, data are representative of n = 1 experiment. Unpaired t test performed. e) Mice were treated as described in Fig. 2h. n = 5-8 mice per group. Data shown are from n = 2 biological replicates. Tumors were analyzed and dendritic cells quantified by flow cytometry. Significance was determined by Brown Forsythe and Welch test with Dunnett’s T3 correction. For d and e, data are presented as mean values +/− SD.
