Extended Data Fig. 10: SAA suppress immunological memory after tumor resection and disrupt systemic immune biology.
From: Hepatocytes coordinate immune evasion in cancer via release of serum amyloid A proteins
a) Study design for b-c. Cured Saa−/− mice after surgical resection of tumor (compared to tumor-naïve mice) were rechallenged with 1e6 7940B tumor cells injected subcutaneously. n = 3 mice per group. b) Overall survival. Significance was determined by Mantel Cox test. c) Tumor growth curves after rechallenge. d) Study design for e-f. Cured mice after tumor rechallenge from a-c (compared to tumor-naïve mice) were depleted of T cells weekly starting one day before challenge with 1e6 subcutaneous 7940B cells. e) Overall survival. Mantel Cox test was performed. f) Mean tumor growth curves after rechallenge. Data shown are representative of 1 biological replicate. g) Ten patients with advanced untreated pancreatic cancer were divided into two groups of n = 5 each by median serum SAA level (215 µg/mL) measured by ELISA. h-i) Saa+/+ or Saa−/− mice (n = 5/group) were orthotopically injected with 5e5 PDA.69 cells. Twenty days later, spleens were collected and analyzed by CyTOF. h) Representative flow plots of CD14+ monocytes and CD3+ T cells. i) Quantification of CD14+ monocytes and CD3+ T cells. Unpaired t tests were performed. j) SAA serum levels detected by ELISA for stage-matched cohorts of treatment-naive, surgically resected short- and long-term survivors (STS, n = 9, and LTS, n = 16). All outliers are shown. Significance was determined by Mann Whitney t-test (two sided). For g, i, and j, data are presented as mean values +/−SD.
