Extended Data Fig. 1: Liver inflammation correlates with low T cell infiltration.
From: Hepatocytes coordinate immune evasion in cancer via release of serum amyloid A proteins
a) Gating strategy used for neutrophil-to-lymphocyte ratio analysis in peripheral blood. b) Mice were implanted with orthotopic PDAC tumors as described in Fig. 1a. FOXP3+ and CD8+ cells in tumors were quantified by immunohistochemistry. CD4+ T cells in tumors and Ly6G+ cells in livers were quantified by flow cytometry. IL-6 serum levels were measured by cytokine bead array. CD3+ and Ly6G+ cells/uL in the peripheral blood (PB) were measured by flow cytometry. Significance was determined by Mann Whitney test with correction for multiple comparisons. n = 19-20 mice per group. c) Six clonal PDAC cell lines (n = 3 T cellhigh and n = 3 T celllow) were chosen based on their similar tumor burden. Mice were analyzed as described in Fig. 1a. d) Tumor weight 20 days post-orthotopic implantation. Mann Whitney test performed. e) Individual mice were clustered based on the variables listed in Fig. 1a. f) Correlation matrix of the variables described in Fig. 1a. g) Percent area of metastatic lesions in livers of mice described in Fig. 1a. Lesions were measured by H&E staining. Mann Whitney test performed. h) Transcriptional expression of tumor markers (Pdx1 and Krt19) in livers of C57BL/6 mice with an orthotopic T cellhigh (655c3, n = 6 mice) or T celllow (6419c1, n = 4 mice) tumor measured by RNA sequencing analysis. Mann Whitney test performed. i-j) Wild-type mice were intraportally injected with 2e5 PDA cells. n = 2 mice per cell line. 14 days later, livers were formalin fixed, paraffin embedded, and stained with hematoxylin & eosin. Metastatic lesions were quantified. Significance was determined by Mann-Whitney test. For violin plots, all data points are shown with lines at the median and quartiles. For h and j, data are presented as mean values +/− SD.
