Extended Data Fig. 3: 10E8 UCA B cells can be activated in vivo, related to Fig. 2.
From: Affinity gaps among B cells in germinal centers drive the selection of MPER precursors
a, Representative equilibrium binding curves of 10E8-GT9.2 interacting with human 10E8-UCAH/UCAL and 10E8-NGS-04H/10E8-NGS-04L as measured by the monomer SPR assay. b, Quantification of precursor frequencies corresponding to transfer of 5,000, 50,000, 100,000, 200,000 and 500,000 CD45.2+10E8-UCAH B cells from donor mice to the host wild-type CD45.1+ mice. (n = 4). Error bars indicate mean ± SD from mice in each group. Experiment performed once. c, Flow cytometry of CD45.2+ cells in splenic GCs of mice transferred with varying numbers of CD45.2+10E8-UCAH precursors and immunized with 50 μg of 10E8-GT9 12mer and the Ribi adjuvant system 7 DPI. d, Representative gating strategy used for the GC response in adoptive transfer experiments.
