Fig. 3: THX mice huTCR cell repertoire and clonality are similar to those in humans.

a, huVα and huJα (huTCRa) genomic representation and gene expression in blood and spleen huT cells of healthy humans (n = 3, HS 05, 06, 07) and non-intentionally immunized THX mice (n = 3, THX mice 369, 370, 371) depicted as stacked columns (left). In these, different colors denote different huVα or huJα gene families; color gradients denote individual family members. Heat maps of expressed individual huVα and huJα genes (right). b, huVβ and huJβ (huTCRb) genomic representation and gene expression in huT cells of HS and THX mice as in a, depicted as stacked columns (left). In these, different colors denote different huVβ or huJβ gene families; color gradients denote individual family members. Heat maps of individual huVβ and huJβ genes (right). c, Associated expression of huVβ and huJβ genes in HS and THX mouse huT cell repertoires as in a, depicted by Circos plots. d, CDR3 length distribution (top) and frequency (bottom) in huT cell recombined huVαJα-Cα (huTCRa) and huVβDJβ-Cβ (huTCRb) transcripts of HS and THX mice as in a. Each dot depicts CDR3 length in an individual cell. e, huT cell clones in HS and THX mice as in a, as identified by unique huVβDJβ-Cβ (including CDR3 as translated amino acid sequence) transcripts and depicted by TreeMaps. Individual rectangle or square (unique color) area reflects huT cell clone size. In THX mice, huVβDJβ-Cβ transcripts identified 5,531, 3,981 and 8,142 discrete huT cell clones in the same order of magnitude as in HS huVβDJβ-Cβ transcripts, which identified 3,437, 11,305 and 4,266 discrete huT cell clones.