Extended Data Fig. 1: PI3Kγ is required for T-dependent antibody responses.
From: PI3Kγ in B cells promotes antibody responses and generation of antibody-secreting cells
(a-b) Serum IgM (a) and IgA (b) levels in Patient A.1. (c-e) Mice were immunized (as in Fig. 1b, c) and antigen-specific IgM (day 5) (c), primary (non-boosted) IgG1 (day 12) (d), or IgG2c (e) was measured via high conjugation ratio NP-BSA ELISA. (f) Mice were immunized (as in Fig. 1d, e) and IgG2b was measured via NP-BSA ELISA. Data are from 2-3 independent experiments and are presented as box-and-whisker plots showing median, min, and max. Data points represent different biological samples. Statistical analysis was performed using non-parametric two-tailed unpaired T-tests. (c) n = 12 for control, n = 12 for KO. (d) n = 15 for control, n = 14 for KO. (e) n = 12 for control, n = 11 for KO. (f) n = 5 for control, n = 6 for KO.
