Extended Data Fig. 2: PI3Kγ is required for GC B cells to adopt ASC gene signatures.
From: PI3Kγ in B cells promotes antibody responses and generation of antibody-secreting cells
(a) Stacked bar plots showing proportions of cell subsets within Naïve or GC B cells used for CSR/SHM analysis. (b) Percentage of high-affinity W38L mutations from the total number of sequences with IGHV1-72 gene and lambda light chains. Statistical analysis was Wilcoxon Rank Sum (c-d) Quantification of marginal zone B cells (c) and follicular B cells (d) from the spleens of indicated mice assessed by flow cytometry. Data are representative of 2 independent experiments (n = 5 for each condition). (e) Volcano plot showing gene expression from RNA sequencing in sorted naïve B cells (IgD+ B220+) from WT versus PI3Kγ KO mice 28 days after SRBC i.p. immunization. Data are from one experiment. (f) Volcano plot of differentially expressed genes from bulk RNA sequencing of sorted GC B cells at day 28 post immunization, as described for Fig. 2e. (g) Expression of ‘Activated B cell’ UPR program genes in sorted splenic GC B cells from Pik3cg heterozygous or KO mice 28 days after SRBC immunization. Transcriptional program is defined by Gaudette, et. al 2020. Data are from one experiment (n = 3 for het, n = 4 for KO). (h) Diagram of germinal center B cells that are beginning to adopt expression of antibody secreting cell surface markers (CD138). Diagram was created with BioRender.com. (c-d) Each dot represents different biological samples and data are presented as box-and-whisker plots showing median, min, and max. Statistical analysis was performed using non-parametric two-tailed unpaired T-tests.
