Extended Data Fig. 7: Di-sulfide linked linked BTN2A1–BTN3A1 ectodomain complexes.
From: Vγ9Vδ2 T cells recognize butyrophilin 2A1 and 3A1 heteromers
(a) Structure of BTN2A1–BTN3A1 depicting the locations of the two cysteine mutant pairs. (b) Predicted structure of the BTN2A1–BTN3A1 complex containing a disulphide bond between BTN2A1 and BTN3A1 molecules, based on the BTN2A1–BTN3A1–zipper ectodomain crystal structure. (c) 2D class averages of negatively stained soluble BTN2A1 Gly102-Cys–BTN3A1 Asp103-Cys ectodomain complex. Each box is 481 Å across.
