Extended Data Fig. 8: Residues in the tail of BTN2A1 are required for pAg-activation of Vγ9Vδ2 T cells.

(a) Surface BTN2A1 expression (clone 259) on HEK293T BTN2A^KO.BTN3A^KO cells that were transfected with BTN2A1 WT or the indicated BTN2A1 intracellular domain mutants, or control BTNL3. Representative from one of two experiments. (b) Representative plots of CD25-PE expression on purified pre-expanded Vδ2⁺ γδ T cells following co-culture with HEK293T BTN2A^KO.BTN3A^KO cells that were co-transfected with BTN3A1 plus the indicated BTN2A1 mutant, or alternatively, control BTNL3 alone or BTN3A1 alone, ± zoledronate (5 µM) for 24 h. Replicates with low transfection efficiency (<10% GFP⁺) were excluded from analysis. Data are from three independent experiments each with n = 2 different donors.