Extended Data Fig. 1: Structure of BTN2A1 in complex with Vγ9Vδ2+ TCR.
From: Vγ9Vδ2 T cells recognize butyrophilin 2A1 and 3A1 heteromers
(a) Unbiased 2mFo-DFc electron density composite omit map contoured at 1σ, blue mesh, surrounding γδTCR-BTN2A1 complex. (b) Size exclusion (S200 16/600) gel filtration chromatogram of BTN2A1 (black) and BTN3A1 (red) ectodomains produced in MGAT1-deficient Expi293F cells. Larger elution volume indicates smaller protein size. Data representative of at least three independent experiments. (c) Unbiased 2mFo-DFc electron density composite omit map contoured at 1σ, blue mesh, surrounding apo BTN2A1 (averaged over five copies of BTN2A1). (d) Overlay of BTN2A1 V-dimer structures derived from the apo (blue) and γδTCR-liganded (green) crystal structures. (e) Overlay of BTN2A1 head-to-tail dimers derived from the apo BTN2A1 (grey) and γδTCR-BTN2A1 (unliganded copy of BTN2A1, red) crystal structures.
