Extended Data Fig. 2: Characterization of GFP-expressing cell types in IL-4-GFP thymi.
a. Gating strategy for characterization of GFP-expressing cell types in IL4-GFP thymi. b. Frequency of GFP+ cells in the indicated thymic subsets in IL4-GFP mice (n = 3 mice). c. Frequency of GFP+ cells among mTECs (gated as EPCAM+CD45–UEA1+Ly51–) in IL4-GFP mice (n = 3 mice). d. Images of IL4-GFP and WT thymi sections as analyzed by confocal immunofluorescence microscopy as in Fig. 3b. An area of the same image as in Fig. 3b is shown for the IL4-GFP mouse. e. Frequency of GFP+ hematopoietic cells in the thymi of mixed BM chimeras shown in Fig. 3c, e. Quantification (left) and representative flow cytometric plots (right) are shown (n = 4 chimeras for [WT+Jedi-TCRαβ]→WT thymi, n = 6 for the other groups). Data are presented as the mean ± s.d.
