Extended Data Fig. 7: Decreased thymic abundance of IL17A-GFP results in autoimmune elimination of GFP+ TH17 cells.
a. Comparison of the frequencies of GFP+ lymphocytes in the thymi, spleens and mLNs of IL17A-GFP mice and in IL17A-GFP→WT BM chimeras (analyzed 8 weeks after reconstitution). One experiment with 2 IL17A-GFP mice and 3 BM chimeras. b–e. WT recipient mice were irradiated and transferred i.v. with T- and NK-cell depleted BM cells from either IL17A-GFP mice, or Jedi-TCRβ mice and WT mice, or Jedi-TCRβ mice and IL17A-GFP mice (all on CB6F1 background). Mesenteric lymph nodes (mLN) (b, d) and thymi (c, e) were analyzed 8 weeks after reconstitution. Same experiment as in Fig. 7a–c. b, c. Frequency and cell surface phenotype of H2-Kd GFP200-208 tetramer-binding CD8 T cells. d, e. Frequency of IL17A-GFP expressing cells among total lymphocytes and among Vβ4– (non-Jedi) CD4 T cells. Representative results of two independent experiments (n = 3 chimeras per group). f. TH17 cells were differentiated in vitro from WT and IL17A-GFP CD4 T cells as in Fig. 7d. Intracellular expression of IL17A cytokine and GFP was analyzed after a short-term PMA/Ionomycin restimulation. Data are presented as the mean ± s.d. with *P < 0.05 and **P < 0.01. Data were analyzed by two-tailed Student’s t test.
