Fig. 7: Decreased thymic abundance of IL-17A–GFP results in autoimmune elimination of peripheral GFP+ TH17 cells.
a, Schematic representation of experiments shown in b–d. WT recipient mice were irradiated and transferred i.v. with T cell- and NK cell-depleted BM cells from IL-17A–GFP mice or Jedi-TCRβ mice and WT mice or Jedi-TCRβ mice and IL-17A–GFP mice (all on a CB6F1 background). Spleens were analyzed 8 weeks after reconstitution. Note the near absence of thymic IL-17A–GFP-expressing cells in BM chimeras (see Extended Data Fig. 7a). b, Frequency and cell-surface phenotype of H2-Kd GFP200–208 tetramer-binding CD8+ T cells in the spleens of BM chimeras. c, Frequency of IL-17A–GFP-expressing cells among total lymphocytes and Vβ4– (non-Jedi) CD4+ T cells. d, Twenty hours before collection, CTV-labeled TH17 cells in vitro differentiated from IL-17A–GFP CD4+ T cells were i.v. transferred into the indicated groups of chimeras. Elimination of the GFPhi fraction of CTV+ cells was assessed by flow cytometry. Representative results of two independent experiments (b and c) or one experiment (d; n = 3 chimeras per group) are shown. Data are presented as mean ± s.d. (*P < 0.05, ***P < 0.001 and ****P < 0.0001) and were analyzed by two-tailed Student’s t-tests.
