Extended Data Fig. 3: Myd88 and Tlr7 deficiency rescues abnormal total splenocyte phenotypes in vikala mice.

a-f, Flow cytometric quantification of proportion and total numbers of activated CD4 T cells (CD3⁺CD4⁺CD44⁺Foxp3^-) (a); and T regulatory cells (Treg; CD3⁺CD4⁺CD44⁺Foxp3⁺) (b) from 20–28 week-old vikala mice Tnip1^+/+ (n = 10), Tnip1^vik/+(n = 11), Tnip1^vik/vik (n = 9); T follicular regulatory cells (Tfr; CD4⁺PD1⁺CXCR5⁺Foxp3⁺) (c) from 22–28 week-old vikala mice; Tnip1^+/+(n = 6), Tnip1^vik/+(n = 14) and Tnip1^vik/vik (n = 7); plasmacytoid dendritic cells (pDCs; CD3⁻CD19⁻CD8⁻CD11c⁺CD11b^-SiglecH⁺BST2⁺) (d) from 20 week-old vikala mice (Tnip1^+/+ (n = 8), Tnip1^vik/+(n = 8), Tnip1^vik/vik (n = 8); and granulocytes (Ly6C⁺ Ly6G⁺CD11b⁺) and monocytes (CD11c^-CD11b⁺Ly6G^-) from 20–28 week-old vikala mice (Tnip1^+/+ (n = 10), Tnip1^vik/+(n = 11), Tnip1^vik/vik (n = 9) (e-f). g-h, Flow cytometric quantification of splenic cell subsets (total number) in 16–20 or 20–30 week-old vikala mice either competent or deficient in MyD88 (g) and Tlr7 (h), respectively. Subsets from left to right; germinal center B cells (GCs; CD19⁺CD95⁺BCL6⁺); age associated B cells (ABC; B220⁺CD21⁻CD23⁻CD19^highCD11c⁺), plasma cells (PC; CD138⁺CD98⁺), T follicular helper cells (Tfh; CD4⁺CXCR5⁺PD1^high), extrafollicular helper T cells (eT_H; CD4⁺CXCR5⁻PD1⁺CXCR3⁺), and switched B cells (B220⁺CD21⁻CD23⁻IgD⁻IgM⁻). Bars represent the median values, and each dot represents an individual mouse (biological replicate) with males and females represented by circles and triangles, respectively (a-h). Data presented in a-b and e-f are all from the same experimental cohort of 20–28 week old vikala mice. Data presented in c and d are each from separate experimental cohorts of 20-week-old and 22–28-week-old vikala mice, respectively. a-h, Results are representative n = 2 experiments. P values were determined by one-way ANOVA with Tukey's test for three-way comparisons following log-transformation of data. ns, not significant.

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