Extended Data Fig. 5: TNIP1Q333P localization with signaling partners and Myddosome component turnover.
From: A TNIP1-driven systemic autoimmune disorder with elevated IgG4
a–h, Immunofluorescence on HEK293 cells for TNIP1 and p62 (green; a), TRAF6 (red; b), A20 (red; c), TAX1BP1 (red, d), LAMP1 (green; e) EEA1 (green; f), RAB7 (green; g) and LC3B (red; h). Scale bars represent 10 μm. Images are representative of n = 3 (b), n = 2 (a, c-f, h) and n = 1 (g) independent experiments. i-j, Immunoblotting of TNIP1 (i), IRAK1 and MYD88 (j) protein in Tnip1+/+ and Tnip1vik/vik BMDMs treated with LPS (100 ng/mL) and Bafilomycin A1 (100 nM) for 2 h. Densitometric plots showing levels of each protein relative to the loading control are shown (right). Data representative of n = 2 (i-j) experiments.
