Fig. 3: TNIP1-driven cellular phenotypes are dependent on MyD88 signaling.

a–f, Representative flow cytometry plots and proportions of splenocytes from male (n = 12, dots) and female (n = 17, triangles) vikala mice aged 16–20 weeks: GC B cells (CD19⁺CD95⁺BCL6⁺) (a); PCs (CD138⁺CD98⁺) (b); ABCs (B220⁺CD21⁻CD23⁻CD19^highCD11c⁺) (c); switched B cells (B220⁺CD21⁻CD23⁻IgD⁻IgM⁻) (d); T_FH cells (CD4⁺CXCR5⁺PD1^high) (e); and extrafollicular T_H cells (CD4⁺CXCR5⁻PD1⁺CXCR3⁺) (f), either competent or deficient in Myd88. Tnip1^+/+ (n = 6), Tnip1^vik/+ (n = 8), Tnip1^vik/vik (n = 6), Tnip1^vik/+ Myd88^−/− (n = 6) and Tnip1^vik/vik Myd88^−/− (n = 3). The bars represent the median values; each point represents an individual mouse (biological replicate). g, serum IgG2c antibodies in 16–20-week-old vikala mice either competent or deficient in Myd88; Tnip1^+/+ (n = 9), Tnip1^vik/+ (n = 9), Tnip1^vik/vik (n = 9), Tnip1^vik/+ Myd88^−/− (n = 6) and Tnip1^vik/vik Myd88^−/− (n = 3). The black lines represents the median; each point represents an individual mouse (biological replicate); sex is indicated by the respective symbol. h, Serum antibodies to DNA (ANAs) in 16–20-week-old vikala mice either competent or deficient in Myd88; Tnip1^+/+ (n = 8), Tnip1^vik/+ (n = 12), Tnip1^vik/vik (n = 9), Tnip1^vik/+ Myd88^−/−(n = 6) and Tnip1^vik/vik Myd88^−/− (n = 3). The bars represent the median; each point represents an individual mouse (biological replicate). In a–f, data are representative of n = 2 independent experiments. In g,h, data are from experiments performed once. Statistical significance was performed using a one-way ANOVA with Tukey’s correction for multiple comparison after log-transformation of data. Exact P values are shown.

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