Fig. 6: TNIP1Q333P has impaired colocalization with the autophagosome markers ATG7, MyD88 and IRAK1.
From: A TNIP1-driven systemic autoimmune disorder with elevated IgG4
a,b,d, Immunofluorescence staining of HEK 293 cells expressing TNIP1WT, TNIP1Q333P or TNIP1D472N (red) and ATG7 (green) (a), or MyD88 (b) or IRAK1 (d) (green). DNA was stained with 4′,6-diamidino-2-phenylindole (DAPI). Scale bar, 10 μm. Data are representative of n = 3 (a,b) or n = 2 (d) independent experiments. Scale bar, 10 μm. c, Quantification of the percentage of TNIP1WT, TNIP1Q333P and TNIP1D472N-expressing cells showing localization (black bar) or lack of localization (white bar) with MyD88 within puncta. Data are from one experiment of n = 21 (WT), n = 32 (Q333P) and n = 35 (D472N). TNIP1-expressing cells were imaged. In c, data are representative of n = 3 independent experiments. e, Anti-TNIP1 or anti-MyD88 IBs of overexpressed and immunoprecipitated TNIP1WT, TNIP1Q333P or MyD88 in HEK 293T cells. In e, data are representative of n = 2 independent experiments. WCE, whole-cell extract.
