Extended Data Fig. 1: TB displayed a broader antitumor therapeutic window than CB-5083.
From: Targeting p97–Npl4 interaction inhibits tumor Treg cell development to enhance tumor immunity
a, Summary of p97-related inhibitors. b, Strep pulldown assay to assess the interaction between p97 and Npl4. Data shown are representative results of two independent experiments. c, Co-IP assay to detect the interaction of p97 with Npl4. Data shown are representative results of two independent experiments. d, Inhibition of cell viability in HGC-27 cells. Data shown are representative results of two independent experiments. e, Inhibition of cell viability in GES-1 cells. Data shown are representative results of two independent experiments. f, mRNA levels of CHOP in the indicated cells that had been treated with TB (upper) or CB-5083 (lower). Data shown are representative results of two independent experiments. g, Schematic illustration of the tumor graft model and treatment with compounds. h, Experimental flow chart of a mouse model for MNNG/H. pylori-induced GC and the treatment with TB, CB-5083 or 5-FU. i, Therapeutic windows and tumor growth inhibition abilities of TB and CB-5083. IC50 values of the compounds are shown. j, Tumor weights of tumor graft models after treatment with TB (intragastrically administered). n = 6 biologically independent samples examined over three independent experiments. k, Tumor weights for MC38-bearing mice that had been treated with TB (intravenously injected, i.v.). n = 4 biologically independent samples examined over two independent experiments. l, Body weights of TB-treated (intraperitoneal administration) mice and control group. n = 4 biologically independent samples examined over two independent experiments. m, Body weights of TB-treated (i.v.) mice and control group. n = 4 biologically independent samples examined over two independent experiments. n, Body weights of TB-treated (25mg/kg, i.v.) mice and control group. n = 6 biologically independent samples examined over two independent experiments. o, Whole blood cell routine test of TB-treated mice and control group from panel n. n = 6 biologically independent samples examined over two independent experiments. Data shown as means ± s.d. (d-f) or means ± s.e.m. (j,k,l,m and n). Data were analyzed for significant differences by performing a one-way ANOVA with Dunnett’s post hoc analysis (f, j and k), or two-way ANOVA with Dunnett’s post hoc analysis (l,m), or two-tailed, unpaired Student’s t-tests (n).
