Extended Data Fig. 2: TB treatment promoted the effector function of CD8+ T cells.
From: Targeting p97–Npl4 interaction inhibits tumor Treg cell development to enhance tumor immunity
a, Apoptosis scores of CD8+ (left) and CD4+ T cells (right) based on the expression of T cell apoptosis-correlated genes from the indicated group. b, Violin plot showing the expression of genes correlated to CD8+ T cell effector function in the indicated group. c, Heatmap showing the expression of genes correlated to CD4+ T cell effector function in the indicated group. d, Flow cytometry analysis showing the proportions of CD4+ and CD8+ T cells among the tumor-infiltrated CD45+ cells from Ctrl and TB-treated mice. e, Proportions (left) and absolute numbers (right) of tumor-infiltrated CD4+ and CD8+ T cells per milligram tumor from Ctrl and TB-treated mice. n = 4 biologically independent samples examined over two independent experiments. f, Gating strategy for tumor-infiltrated CD8+ T cells and representative flow cytometry plots of unstimulated samples of the indicated cytokine-producing CD8+ T cells and samples of these cells stimulated with PMA and ionomycin. g, Proportions of tumor-infiltrated IFNγ+ CD8+ T cells per milligram tumor from Ctrl and TB-treated mice. n = 6 biologically independent samples examined over two independent experiments. Data shown as means ± s.e.m. (e,g). Data were analyzed for significant differences by performing two-tailed, unpaired Student’s t-tests (a), or one-way ANOVA with Dunnett’s post hoc analysis (b,e,g).
