Extended Data Fig. 10: Effects of dexamethasone on TFH and myeloid cells. | Nature Immunology

Extended Data Fig. 10: Effects of dexamethasone on TFH and myeloid cells.

From: Arid1a-dependent canonical BAF complex suppresses inflammatory programs to drive efficient germinal center B cell responses

Extended Data Fig. 10

a and b. Quantification of frequencies and numbers of Tfh cells gated on CD45.1 CD4+ cells in Arid1a fl/fl and Cg1 Arid1aKO chimeras at day 11 post-SRBC immunization. Arid1a fl/fl and Cg1 Arid1aKO chimeras treated with DMSO are open bars and Dexa treated groups are represented with light red filled bars. c and d. Quantification of frequencies and numbers of CD11b expressing myeloid cells gated on CD45.1 cells in Arid1a fl/fl and Cg1 Arid1aKO chimeras at day 11 post-SRBC immunization. Arid1a fl/fl and Cg1 Arid1aKO chimeras treated with DMSO are open bars and Dexa treated groups are represented with light red filled bars. e and f. Quantification of frequencies and numbers of Ly6G- Ly6Chi monocytes gated on CD45.1+ CD11b expressing myeloid cells in Arid1a fl/fl and Cg1 Arid1aKO chimeras at day 11 post-SRBC immunization. g and h. Quantification of frequencies and numbers of Ly6G+ Ly6Clo neutrophils gated on CD45.1+ CD11b expressing myeloid cells in Arid1a fl/fl and Cg1 Arid1aKO chimeras at day 11 post-SRBC immunization. i. Quantification of splenocyte numbers in Arid1a fl/fl and Cg1 Arid1aKO chimeras at day 11 post-SRBC immunization with DMSO or Dexa treatment. Statistical significance is calculated using 2-way ANOVA with multiple comparisons. Error bars represent mean ± s.e.; *p value ≤ 0.05; **p value ≤ 0.01; ***p value ≤ 0.0005; ****p value < 0.0001.

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