Extended Data Fig. 2: Characterization of peripheral B cell compartment in CD19 Arid1a KO mice.

a. Representative flow cytometry analysis of mature (AA4.1- B220+) and immature B cells (AA4.1+ B220+) subsets from splenic B cells in Arid1a fl/fl (YFP-) and CD19 Arid1a KO (YFP+) mice. b. Quantification of splenic mature and immature B cell subsets from Arid1a fl/fl (YFP-; n = 5) and CD19 Arid1a KO (YFP+; n = 5) mice. c and d. Quantification of splenic Follicular (FO) B cells (IgD hi, IgM low) and Marginal zone (MZ) B cells (IgD low, IgM hi) from Arid1a fl/fl (YFP-; n = 5) and CD19 Arid1a KO (YFP+; n = 5) mice. e. Representative flow cytometry analysis showing YFP expression in B cells (left panel) and GC B cells (right panel) from Peyer’s patches of CD19 Arid1aKO mice. f. Representative flow cytometry analysis showing YFP reporter expression in GC B cells (middle panel) and non-GC B cells (right panel) from SRBC immunized Cg1 cre Rosa26 loxp-stop-loxp YFP reporter mice. g. Representative flow cytometry analysis of splenic GC B cells gated on B cells from NP-Ova immunized Arid1a fl/fl, Cg1 Arid1a fl/+(Het) and Cg1 Arid1a KO mice at 14 days post-immunization. h. Quantification of GC B cell frequency in spleen of Control (Ctrl) (both Arid1a fl/fl and Cg1 cre; n = 12), Cg1 Arid1a fl/+(Het) (n = 4) and Cg1 Arid1a KO (n = 6) mice. i. Schematic of a flow cytometry-based assay to quantify SRBC-specific IgM and IgG1 antibodies in the sera. j and k. Quantification of serum SRBC-specific IgM and IgG1 antibodies upon SRBC immunization in Ctrl (Arid1fl/fl and Cg1 Cre; n = 4) and Cg1 Arid1aKO mice (n = 4). Statistical significance is calculated using multiple unpaired t-tests for b and two-tailed Student’s t-test for c, d, j and k, and one-way ANOVA for h. Error bars represent mean ± s.e.; *p value ≤ 0.05; **p value ≤ 0.01; ***p value ≤ 0.0005; ****p value < 0.0001.