Extended Data Fig. 1: Identification of the NaCl effective dose on CD8+ T cells and evaluation of osmotic pressure.
From: NaCl enhances CD8+ T cell effector functions in cancer immunotherapy
a, Representative flow cytometry gating strategy to identify human CD8+ T cells. b, Mean ± s.e.m. % viable CD8+ T cells recovered at day 5 after activation of cells as in Fig. 1a and after treatment with increasing concentrations of NaCl (0 mM, n = 10; 20 mM, n = 3; 40 mM, n = 9; 60 mM, n = 3; 80 mM, n = 14; 100 mM, n = 2; 150 mM, n = 4). c, Mean ± s.e.m. summary of the levels (MFI) of CD27, GZMB, PD-1, TIM3 and T-bet on CD8+ T cells after activation with anti-CD3/28 + IL-2 in control medium (Ctrl) and with increasing concentrations of NaCl (n = 4 donors except 20 mM NaCl n = 2; two independent experiments; Kruskal-Wallis test). d, Representative flow cytometry analysis of % GZMB and T-bet expression in CD8+ T cells activated as in b and treated with excess NaCl (80 mM) or equiosmolar urea (176 mM final concentration) or mannitol (142 mM final concentration). e, Mean ± s.e.m. summary of data shown in d (n = 2, mannitol; n = 4, all other conditions; two independent experiments; Kruskal-Wallis test). f, As in e, but related to PD-1, TIM3 and CD27 expression (two independent experiments; Kruskal-Wallis test).
