Extended Data Fig. 2: NaCl potentiates TCR signaling and acts in part via SGK1.
From: NaCl enhances CD8+ T cell effector functions in cancer immunotherapy
a, Representative flow cytometry of % pERK, pS6, and GZMB expression by CD8+ T cells activated as in Fig. 1a and restimulated at day 5 with plate-boundanti-CD3 and soluble anti-CD28 for 30 min. b, Mean ± s.e.m summary of the data as in a (indicated as % expression of the marker among total CD8+; n = 6, two independent experiments; two-sided paired t-test). c, Schematic representation of the experiment in d. d, Mean ± s.e.m summary of flow cytometric analysis of specific (combinations of) markers by Ctrl or NaCl-treated CD8+ T cells (n = 3, one experiment; two-sided paired t-test). Data are expressed as % expression or median fluorescence intensity (MFI) of the marker among total CD8+ T cells. e, Summary of flow cytometric analysis of specific markers by CD8+ T cells activated as in Fig. 1a but in the presence or 2μM SGK1 inhibitor GSK 650394 (n = 6, two independent experiments; paired one-way ANOVA with Dunn’s post test). Data are expressed as MFI.
