Extended Data Fig. 3: High-salt diet increases the infiltration of CD8⁺, CD4⁺ and NK cells in tumors without altering the frequency of CD4⁺ Treg cells.

a, Mean ± s.e.m. CD4⁺ and CD8⁺ T cell count/gram of MC38 tumor and b, absolute count of CD4⁺ and CD8⁺ T cells in the spleen of tumor-bearing B6 male mice fed with normal salt diet (NSD, n = 16) or high-salt diet (a: HSD, n = 15; b, HSD, n = 14; in b, one spleen sample was removed because of faulty antibody staining). Data are from three independent experiments, two-sided unpaired t-test. c, Frequency of T_CM (central memory, CD44^hiCD62L^hi), T_EM (effector memory, CD44^hiCD62L^lo) and T_TE (terminal effectors, CD44^loCD62L^lo) among total CD8⁺ T cells in the tumor (NSD, n = 11; HSD, n = 12) and d, of FOXP3⁺ cells among CD4⁺ T cells in the spleen and in the tumor of B6 male mice fed as in a (NSD, n = 10; HSD, n = 6). Data are from two independent experiments; two-sided unpaired t-test. Data from one experiment was excluded due to faulty FOXP3 antibody staining. e. NK cell count/gram of MC38 tumor from B6 male mice fed as in a (NSD, n = 16; HSD, n = 15; three independent experiments; two-sided unpaired t-test).

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