Extended Data Fig. 1: Tracking a Listeria-specific CD8+ T cell response against LADD by TCR use and phenotyping with CyTOF.
From: A mass cytometry method pairing T cell receptor and differentiation state analysis
(a) Gating strategy for all CyTOF analyses preceding input into semi-supervised Vα-Vβ assignment script. (b) Representative flow plots and quantification of roliferating CD8+ T cells from spleens 5 days after LADD, LADD-OVA, or PBS injection IV. P values (from top to bottom) 0.0286, 0.0159. (c) Bar plot of Vα2, Vβ14, and Vα2_Vβ14 percentages in each cluster for conditions LAAD and LAAD-OVA. (d) UMAP visualization of pooled CD8+ T cells colored by the expression of Vβ12+ in LADD, LADD-OVA, or PBS infected mice. Results from n=5 for PBS, n=4 for LADD, n=4 for LADD-OVA, P values were calculated by unpaired two-tailed student’s T-test, mean ± s.e.m.
