Extended Data Fig. 2: Tracking Listeria-specific CD4+ T cells against LADD by TCR use and phenotyping with CyTOF.
From: A mass cytometry method pairing T cell receptor and differentiation state analysis
(a) Representative flow plots and quantification of proliferating (Ki-67+) CD4+ T cells from spleen 5 days after LADD, LADD-OVA, or PBS injection. P values (from top to bottom) 0.0159, 0.0286. (b) UMAP visualization of CD4+ T conventional (non-Treg) cell clusters based on expression of non-TCR proteins. (c) Heatmap of non-TCR protein expression annotated by cluster and fraction of cells falling into each cluster. (d) UMAP visualization of CD4+ T cells colored by the expression Ki-67 in LADD-infected mice. (e) Frequency of Ki-67+ and Ki-67- CD4+ T cells using specific TCR Vβ (left) or Vα chains (right) in response to PBS, LADD, or LADD-OVA. (f-h) Frequency of Vβ13+ (f), Vβ14+ (g), or Vβ13+Vα2+ among Ki-67+(h) versus Ki-67- CD4+ T cells in LADD or LADD-OVA infected mice. P values (from left to right) 0.0286, 0.0079, 0.0286, 0.0159, 0.0159, 0.0286, 0.0079, 0.0159, 0.0286. (i-k) UMAP visualization of pooled CD4+ T cells colored by the expression of Vβ13+ (i), Vβ13+Vα2+ (j), and Vβ14 (k) in LADD, LADD-OVA, or PBS injected mice. (m) Bar plot of Vβ13+, Vβ13+Vα2+, and Vβ14+ percentages in Teff clusters. Results from n=5 for PBS, n=4 for LADD, n=4 for LADD-OVA, P values were calculated by unpaired two-tailed Student’s t-test, mean ± s.e.m.
