Extended Data Fig. 1: LRRK2 is not detected in Paneth cells from EGFP-Lrrk2KI/KI mice.
From: LRRK2 is not required for lysozyme expression in Paneth cells
a) Spleens were dissected from Lrrk2+/+ and Lrrk2-/- littermate mice used to generate ileal organoids shown in Fig. 1b–d, and snap frozen until use. 40 µg of homogenized lysates were loaded and Immunoblotting done for LRRK2 pSer935, LRRK2, total Rab10, pT73 Rab10 and loading controls α-tubulin are shown (n = 2 mice per genotype). A549 cell lysates were used as controls as in Fig. 1c. b) Representative flow cytometric plots showing the gating strategy to identify CD24+CD45– epithelial cells (includes Paneth cells), CD24–CD45–EpCAM+SSClo epithelial cells, and CD24+CD45+ cells (immune cells) isolated from ileal crypts. Graph shows EGFP-LRRK2 geometric mean fluorescence intensities above background in EGFP-Lrrk2KI/KI mice, calculated by subtracting the mean autofluorescence of the respective cell type measured in WT littermate mice (n = 4). Means and individual biological replicates shown. Data representative of 2 experiments.
