Extended Data Fig. 2: Cellular phenotype and source of IL-10 driving Th2 cells in the lung. | Nature Immunology

Extended Data Fig. 2: Cellular phenotype and source of IL-10 driving Th2 cells in the lung.

From: Spatial microniches of IL-2 combine with IL-10 to drive lung migratory TH2 cells in response to inhaled allergen

Extended Data Fig. 2

a, Representative flow plots (a, left) and percentage (a, right, n = 17 in CRISPRcontrol, n = 14 in CRISPRSTAT3) of Blimp-1 YFP+ cells within Th2 cells in the lung. b-e, flow cytometry analysis of Th2 cells(gated on live TCRβ + CD4+FoxP3-) in the lung (b) isolated from control (IL-10f/fCD11cCre−) or IL-10CD11cCre (IL-10f/fCD11cCre+) animals i.n. immunized with HDM. c-d, percent of infiltrating eosinophils (c) and monocytes (d) in the BAL. e, Percent of effector CD4 + T cells expressing Blimp-1 in the lung (gated on live TCRβ + CD4+FoxP3-). Blimp-1f/fCD4Cre+ (Blimp-1 cKO) mice were used as gating control for Blimp-1 detection. b-e, control (IL-10f/fCD11cCre−, n = 19), IL-10CD11cCre (IL-10f/fCD11cCre+, n = 18). f-i, flow cytometry analysis of Th2 cells (gated on live TCRβ + CD4+FoxP3-) in the lung (f) isolated from control (IL-10f/fCD19Cre−) or IL-10CD19Cre (IL-10f/fCD19Cre+) animals i.n. immunized with HDM. g, percent of infiltrating eosinophils (g) and monocytes (h) in the BAL. i, Percent of effector CD4 + T cells expressing Blimp-1 in the lung (gated on live TCRβ + CD4+FoxP3-). Blimp-1 cKO mice were used as gating control for Blimp-1 detection. For f, i, control (IL-10f/fCD19Cre−, n = 16) or IL-10CD19Cre (IL-10f/fCD19Cre+, n = 20). For g-h, control (IL-10f/fCD19Cre−, n = 11) or IL-10CD19Cre (IL-10f/fCD19Cre+, n = 13). j, number of infiltrating monocytes in the BAL from control (IL-10f/fCD4Cre−, n = 18) or IL-10CD4Cre (IL-10f/fCD4Cre+, n = 15) animals i.n. immunized with HDM. k-l, experimental setup of naïve IL-10CD4Cre-1DERYFP (CD45.1/2+) CD4 + T cells transferred into IL-10 KO (CD45.2+) recipients followed by 5d i.n. HDM immunization. Flow analysis (k) and absolute cell number of Th2 cells (l, left, n = 4) and Blimp-1 YFP+ expression within Th2 cells (l, right, n = 4) isolated from lung after HDM. m, experiment diagram for 50:50 mixes of 1DERYFP T cells for data in Fig. 2 (k-o). Each point represents one individual sample. Data are shown as means ± SD above and presented two independent experiments (l) or three independent experiments (a, b-e, f-i, j). Two-tailed unpaired Mann-Whitney t test (a, l). a: ****P < 0.0001; l (left): p = 0.0286, l (right): p = 0.0286.

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