Extended Data Fig. 4: CITE-seq and response with CCR8 deficiency.
a, UMAP visualization to identify labeled CD45.1 + 1DER T cells. b, Pseudotime trajectory analysis of CD4 T clusters. c, Pseudotime trajectory analysis using Slingshot assay. d-g, Naïve 1DERYFP (CD45.1/2+) CD4 + T cells were electroporated with CRISPR sgRNA/Cas9 RNPs targeting CCR8 or scramble control and immediately transferred into WT (CD45.2+) recipients immunized (24 h later) with HDM for 5d. (d, f) flow cytometry plots, absolute number of Th2 cells (e, n = 11) and (g, n = 11) Blimp-1 YFP+ cells within Th2 cells from 1DERYFP T cells in the lung. Data are shown as means ± SD and presented two independent experiments. Two-tailed unpaired Mann-Whitney t test (e, g), p > 0.05.
