Extended Data Fig. 4: Immunofluorescence confirmation of cardiac troponin T expression in induced pluripotent stem cell-derived cardiomyocytes (iPSC-CM).
From: UBXN9 governs GLUT4-mediated spatial confinement of RIG-I-like receptors and signaling
a, Timeline and experimental protocol for the generation of induced pluripotent stem cells (iPSCs), differentiation into iPSC-derived cardiomyocytes (iPSC-CMs) and subsequent treatment conditions for assessing RLR responses. CHIR99021, GSK3 inhibitor/Wnt activator; IWP-4, inhibitor of Wnt production-4. b, Confocal microscopic images of cardiac troponin T (cTnT) counterstained with DAPI (nuclear DNA) in iPSC-CM on day 35 of differentiation. Top panel, 20x magnification; bottom panel, 100x magnification. Scale bar, 100μm (top) and 25μm (bottom).
