Extended Data Fig. 1: UBXN9 is critical for RLR signaling in vitro and in vivo.
From: UBXN9 governs GLUT4-mediated spatial confinement of RIG-I-like receptors and signaling
a, Immunoblots of UBXN9 protein in Ubxn9+/+ and Ubxn9−/− C2C12 myocytes. b, c, Cellular Ifnb1 mRNA levels (n = 4 biological replicates/group) (b) and secreted IFN-β protein (12 h, n = 2 biological replicates/group; 24 h, n = 4 biological replicates/group) (c) from C2C12 myocytes transfected with 3p-hpRNA. d, Secreted IFN-β protein from C2C12 myocytes (n = 2 biological replicates) transfected with interferon-stimulatory DNA (ISD). e-f, Secreted IFN-β protein (e) and viral RNA loads (f) in mouse C2C12 cells (n = 4 biological replicates/group) infected with ONNV (MOI: 1). g-h, Ubxn9+/+ and Ubxn9−/− littermates (n = 5 Ubxn9+/+ and 6 Ubxn9−/− mice) infected with ONNV as depicted in (g), and viral RNA in hindlimb muscles (h). Dpi: days post infection. The data are representative of 2-3 independent experiments (a-c, e, f) or 1 (d, g,h) independent experiment. Bar: mean ± SEM. P values were determined by by two-way ANOVA with Šídák multiple comparisons test (b-f) or two-tailed Mann-Whitney test (h). LOD: limit of detection, established with uninfected mice (h).
