Extended Data Fig. 3: GLUT4 inhibits RLR signaling in C2C12 myocytes.
From: UBXN9 governs GLUT4-mediated spatial confinement of RIG-I-like receptors and signaling
a, Mean fluorescence of VSV-GFP+ in Slc2a4+/+ and Slc2a4−/− C2C12 cells (N = 4 biological replicates/group) at 24 h post VSV infection (MOI: 1). b, VSV-G protein in Slc2a4+/+ and Slc2a4−/− C2C12 cells before and after VSV infection (MOI: 1). c, d, EMCV titers (c) and IFN-β protein levels (d) from Slc2a4+/+ and Slc2a4−/− C2C12 cells (c, n = 4 biological replicates/group; d, n = 2-4 biological replicates/group) infected with EMCV (MOI: 0.5). e, f, Phosphorylated (p) and total IRF3 before and after ONNV (e) or EMCV (f) infection in C2C12 cells. EMCV MOI: 0.5; ONNV MOI: 1. The data are representative of 2 independent experiments. Bar: mean ± SEM. P values determined by two-tailed Student’s t-test (a) or two-way ANOVA with Šidák’s multiple comparisons test (c, d).
