Extended Data Fig. 7: Contribution of +6.5kbIl13 during chronic lung inflammation, related to Fig. 6.
From: Remodeling of Il4-Il13-Il5 locus underlies selective gene expression
a, mRNA expression of Il4, Il13 and Il5 in WT and +6.5kbIl13 KO ILC2s stimulated with IL-33 + NMU measured by qPCR. The data were normalized to Actb, then further normalized to non-stimulated WT cells ( = 1). Graphs are representative plots from three independent experiments, and triplicates summary are shown. b-d, WT (n = 13) and +6.5kbIl13−KO (Δ+6.5kbIl13, n = 10) mice were challenged with papain to induce lung inflammation. c, Flow cytometry for infiltrating cells recovered from the lungs. Cells evaluated were total live cells, eosinophils (CD11b+ Siglec-F+), neutrophils (CD11b+ Gr1+), Th2 (CD3ε+ TCRβ+ CD4+ CD44+ Foxp3- GATA3+) and ILC2s (Lin− Thy1+ CD127+ GATA3+). d, Cytokine production from Th2 cells was evaluated by flow cytometry measuring IL-4, IL-13 and IL-5. Graphs in c and d show mean ± SEM. Statistical significance is depicted as ∗p < 0.05, ∗∗p < 0.01 and ∗∗∗p < 0.001 (Two-sided t test).
