Extended Data Fig. 1: Gene expression, TF loading, and in situ Hi-C of ILC2s and Th2 cells, related to Fig. 1.
From: Remodeling of Il4-Il13-Il5 locus underlies selective gene expression
a and b, Gene expression of transcription factors in mouse Th2 and ILC2s by mRNA-seq (GSE131996). c, Aggregated NFAT1 load near HIGs (+/-50kb from TSS) were calculated and binned into 3 categories (common (n = 45), ILC2 specific (n = 70) or Th2 specific (n = 49)) and graphed (Y-axis) for 4 sample conditions (X-axis), as in Fig. 1d. The lower and the upper edge of the box represent the first percentile and the third percentile of the data, respectively. The line inside the box represents the median. The whiskers represent the minimum, maximum and its variability in comparison to the interquartile range. A two-sided t test was used to statistically evaluate the difference of the NFAT1 load between ILC2 specific HIGs and Th2 specific HIGs. d, Interaction frequency (Y axis) vs distance (X axis) of chromatin, measured by HiC for 8 samples. e, Percentage of local chromatin interactions. f, Percentage of interchromosomal interactions. g, Number of TADs detected in individual samples. All TADs are merged by merge2Dbed.pl function by HOMER for downstream analysis.
