Extended Data Fig. 3: Detection of circulating antigen-specific Igs antibodies upon vaccination.

a, Cartoon showing the Luminex bead-based assay. b, Nucleocapsid IgG threshold used to define SARS-CoV-2 negative samples. Each dot represents a sample tested. Boxplots represent min to max and show all points. Statistics was calculated with non-parametric Kruskal-Wallis with Dunn’s multiple comparisons c, IgM and d, IgA reactivity to the SARS-CoV-2 proteins NTD, S2, S1 and RBD and percentage of responders in HD and SLE. Each dot represents a sample tested. Negative values are based on MFI values of time 0 (T0) from pre-pandemic and vaccine baseline samples, and are indicated below the gray area. Percentage of positive responders above the gray area is shown as a numberical value above each tested time-point e, Kinetics analyses for IgG binding to S1, RBD, S2, and NTD proteins by Luminex assay. Overlay of geometric mean and pie charts showing the proportion of negative, low, medium, or high values. Statistics of the geomean between HD and SLE were performed with two-sided U Mann Whitney for comparison of each indicated time. Pie chart comparison statistics performed with Chi-square with Fisher test analysis. f, ELISA detection of IgG reactive to beta-common cold coronaviruses (beta-CoV) HKU-1 and OC43 and alpha-common cold coronaviruses (alpha-CoV) NL63 and 229E, tested with sera from pre-vaccinated (baseline) donors enrolled in the study. Boxplots represent median with interquartile range, and whiskers indicate range. Each dot represents a tested sera sample from n = 8 HD and n = 9 SLE at baseline. Statistical comparison was performed with unpaired t-test. Not significant, ns p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.