Extended Data Fig. 4: Detection of circulating neutralizing Igs upon vaccination and quantitation of anti-spike B cells. | Nature Immunology

Extended Data Fig. 4: Detection of circulating neutralizing Igs upon vaccination and quantitation of anti-spike B cells.

From: Disease-associated B cells and immune endotypes shape adaptive immune responses to SARS-CoV-2 mRNA vaccination in human SLE

Extended Data Fig. 4

a, Cartoon showing the steps of pseudoviral in vitro neutralization assay. b, NT50 values detected from HD and SLE plasma and comparison of all viral strains for each sample. Fold changes show the difference between Delta and Omicron compared to the WT values. c, Circulating CD19+ B cells frequency comparison in our cohorts. d, Correlation of CD19+ frequency and spike reactive B cells in HD and SLE for each vaccine time point. Blue lines show the range observed in the HD. Pearson analysis was performed for each comparison. Quantification of total (e) spike-specific and (f) RBD-specific B cells shown as the frequency of viable lymphocytes in the HD and SLE cohorts. Each dot represents an individual sample tested at baseline (d0) and after receiving one (Vax 1), two (Vax 2), or three (Vax 3) vaccine doses. Pie charts comparisons and Chi-square with Fisher’s test are shown. When indicated, LOD=limit of detection set to logarithmic 0.001 for B cells and 0.003 for T cells. LOS=limit of sensitivity, based on median values of baseline + 2 x SD. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

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